Hydrophobic recognition allows the glycosyltransferase UGT76G1 to catalyze its substrate in two orientations

Ting Yang; Jinzhu Zhang; Dan Ke; Wenxian Yang; Minghai Tang; Jian Jiang; Guo Cheng; Jianshu Li; Wei Cheng; Yuquan Wei; Qintong Li; James H. Naismith; Xiaofeng Zhu

doi:10.1038/s41467-019-11154-4

Nature Communications (Jul 2019)

Hydrophobic recognition allows the glycosyltransferase UGT76G1 to catalyze its substrate in two orientations

Ting Yang,
Jinzhu Zhang,
Dan Ke,
Wenxian Yang,
Minghai Tang,
Jian Jiang,
Guo Cheng,
Jianshu Li,
Wei Cheng,
Yuquan Wei,
Qintong Li,
James H. Naismith,
Xiaofeng Zhu

Affiliations

Ting Yang: Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Jinzhu Zhang: Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Dan Ke: Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Wenxian Yang: Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Minghai Tang: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Jian Jiang: Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Guo Cheng: West China School of Public Health, Healthy Food Evaluation Research Center and State Key Laboratory of Biotherapy and Cancer Center, Sichuan University
Jianshu Li: Department of Biomedical Polymers and Artificial Organs, College of Polymer Science and Engineering and State Key Laboratory of Polymer Materials Engineering, Sichuan University
Wei Cheng: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Yuquan Wei: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Qintong Li: Department of Pediatrics, Obstetrics and Gynecology, West China Second University Hospital, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, Sichuan University
James H. Naismith: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Xiaofeng Zhu: Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University

DOI: https://doi.org/10.1038/s41467-019-11154-4
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 12

Abstract

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UGT76G1 is an UDP-glucose dependent glycosyltransferase and a component of the biosynthesis pathway for the natural sugar substitute steviol glycoside. Here, the authors present substrate bound crystal structures of UGT76G1 and provide insights into substrate recognition and catalysis by the enzyme.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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