Damage to <i>Sorubim cuspicaudus</i> Sperm Cryopreserved with Ethylene Glycol
Víctor Atencio-García,
Denia Padilla-Izquierdo,
Juana Robles-González,
Martha Prieto-Guevara,
Sandra Pardo-Carrasco,
José Espinosa-Araujo
Affiliations
Víctor Atencio-García
CINPIC, Fishculture Research Institute, School of Veterinary Medicine and Zootechnics, Department of Aquaculture Sciences, University of Córdoba, Montería 230002, Colombia
Denia Padilla-Izquierdo
School of Basic Sciences, Department of Biology, University of Córdoba, Montería 230002, Colombia
Juana Robles-González
School of Basic Sciences, Department of Mathematics and Statistics, Universidad de Córdoba, Montería 230002, Colombia
Martha Prieto-Guevara
CINPIC, Fishculture Research Institute, School of Veterinary Medicine and Zootechnics, Department of Aquaculture Sciences, University of Córdoba, Montería 230002, Colombia
Sandra Pardo-Carrasco
School of Agricultural Sciences, Department of Animal Husbandry, National University of Colombia, Medellín 050034, Colombia
José Espinosa-Araujo
CINPIC, Fishculture Research Institute, School of Veterinary Medicine and Zootechnics, Department of Aquaculture Sciences, University of Córdoba, Montería 230002, Colombia
The study aimed to evaluate cryo-injury during the cryopreservation in Sorubim cuspicaudus sperm with ethylene glycol (EG) at different rates (6, 8, 10%). Fresh, prefrozen, and post-thawed sperm quality as motility total, velocities, mitochondria damage (Mit-d), membrane damage (Mem-d), and DNA fragmentation (DNA-f), were examined. The Mit-d, Mem-d, and DNA-f were evaluated through flow cytometry. High motility (>95%) and a low percentage of Mem-d (1.0 ± 0.5%), Mit-d (1.4 ± 0.9%), and DNA-f (2.4 ± 0.8%) were recorded for fresh semen. Prefrozen semen increases in Mit-d and DNA-f were observed compared to fresh semen (p S. cuspicaudus semen.
