Acta Biomaterialia Odontologica Scandinavica (Dec 2016)

Antimicrobial properties and dental pulp stem cell cytotoxicity using carboxymethyl cellulose-silver nanoparticles deposited on titanium plates

  • Martha Alicia Laredo-Naranjo,
  • Roberto Carrillo-Gonzalez,
  • Myriam Angelica De La Garza-Ramos,
  • Marco Antonio Garza-Navarro,
  • Hilda H. H. Torre-Martinez,
  • Casiano Del Angel-Mosqueda,
  • Roberto Mercado-Hernandez,
  • Roberto Carrillo-Fuentevilla

DOI
https://doi.org/10.3109/23337931.2016.1160783
Journal volume & issue
Vol. 2, no. 1
pp. 60 – 67

Abstract

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Objective: To evaluate the antimicrobial properties and dental pulp stem cells (DPSCs) cytotoxicity of synthesized carboxymethyl cellulose-silver nanoparticles impregnated on titanium plates. Material and methods: The antibacterial effect of silver nanoparticles in a carboxymethyl cellulose matrix impregnated on titanium plates (Ti-AgNPs) in three concentrations: 16%, 50% and 100% was determined by adding these to bacterial cultures of Streptococcus mutans and Porphyromonas gingivalis. The Ti-AgNPs cytotoxicity on DPSCs was determined using a fluorimetric cytotoxicity assay with 0.12% chlorhexidine as a positive control. Results: Silver nanoparticles in all concentrations were antimicrobial, with concentrations of 50% and 100% being more cytotoxic with 4% cell viability. Silver nanoparticles 16% had a cell viability of 95%, being less cytotoxic than 0.12% chlorhexidine. Conclusions: Silver nanoparticles are a promising structure because of their antimicrobial properties. These have high cell viability at a concentration of 16%, and are less toxic than chlorhexidine.

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