mSystems (Oct 2021)

MetaFunPrimer: an Environment-Specific, High-Throughput Primer Design Tool for Improved Quantification of Target Genes

  • Jia Liu,
  • Paul Villanueva,
  • Jinlyung Choi,
  • Santosh Gunturu,
  • Yang Ouyang,
  • Lisa K. Tiemann,
  • James R. Cole,
  • Kathryn R. Glanville,
  • Steven J. Hall,
  • Marshall D. McDaniel,
  • Jaejin Lee,
  • Adina Howe

DOI
https://doi.org/10.1128/mSystems.00201-21
Journal volume & issue
Vol. 6, no. 5

Abstract

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Amplification-based gene characterization allows for sensitive and specific quantification of functional genes. There is often a large diversity of genes represented for functional gene groups, and multiple primers may be necessary to target associated genes.