mSystems
(Oct 2021)
MetaFunPrimer: an Environment-Specific, High-Throughput Primer Design Tool for Improved Quantification of Target Genes
Jia Liu,
Paul Villanueva,
Jinlyung Choi,
Santosh Gunturu,
Yang Ouyang,
Lisa K. Tiemann,
James R. Cole,
Kathryn R. Glanville,
Steven J. Hall,
Marshall D. McDaniel,
Jaejin Lee,
Adina Howe
Affiliations
Jia Liu
Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, USA
Paul Villanueva
Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, USA
Jinlyung Choi
Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, USA
Santosh Gunturu
Center for Microbial Ecology, Michigan State University, East Lansing, Michigan, USA
Yang Ouyang
Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, Michigan, USA
Lisa K. Tiemann
Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, Michigan, USA
James R. Cole
Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, Michigan, USA
Kathryn R. Glanville
Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, USA
Steven J. Hall
Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa, USA
Marshall D. McDaniel
Department of Agronomy, Iowa State University, Ames, Iowa, USA
Jaejin Lee
Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, USA
Adina Howe
ORCiD Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, USA
DOI
https://doi.org/10.1128/mSystems.00201-21
Journal volume & issue
Vol. 6,
no. 5
Abstract
Read online
Amplification-based gene characterization allows for sensitive and specific quantification of functional genes. There is often a large diversity of genes represented for functional gene groups, and multiple primers may be necessary to target associated genes.
WeChat QR code
Close
