OncoTargets and Therapy (2019-01-01)

MicroRNA-505 suppresses gastric cancer cell proliferation and invasion by directly targeting Polo-like kinase-1

  • Dang SC,
  • Wang F,
  • Qian XB,
  • Abdul M,
  • Naseer QA,
  • Jin W,
  • Hu R,
  • Gu Q,
  • Gu M

Journal volume & issue
Vol. Volume 12
pp. 795 – 803

Abstract

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Sheng-Chun Dang,1 Fei Wang,1 Xiao-Bao Qian,1 Malik Abdul,1 Qais-Ahmad Naseer,1 Wei Jin,2 Rong Hu,3 Qian Gu,3 Min Gu4 1Department of General Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu Province 212001, People’s Republic of China; 2Department of Obstetrics and Gynecology, The Changshu No. 2 People’s Hospital, Changshu, Jiangsu Province 215500, People’s Republic of China; 3Department of Geriatrics, Zhenjiang First People’s Hospital, Jiangsu Province 212001, People’s Republic of China; 4Department of Oncology, Zhenjiang Hospital of Traditional Chinese and Western Medicine, Zhenjiang, Jiangsu 212001, People’s Republic of China Purpose: The expression of microRNA-505 (miR-505) has been investigated in various cancers; however, its effect and mechanism in relation to gastric cancer (GC) are yet to be determined. Thus, the current evaluation aimed to examine the expression and potential role of miR-505 in GC. Materials and methods: Quantitative real-time PCR was carried out to analyze miR-505 expression in GC cells and tissues. We observed that miR-505 is differentially expressed in GC cells following transfection of its mimics or inhibitors. Changes in cell invasion, cell proliferation, and epithelial–mesenchymal transition markers were measured. Results: These findings indicated that miR-505 expression is downregulated in both GC cell lines and GC tissues. In addition, knockdown miR-505 induced the invasion and proliferation of GC cells. Transfection of miR-505 mimics led to an elevation in N-cadherin expression but a decrease in E-cadherin expression. Furthermore, we have shown that miR-505 binds to the 3'-UTR region of Polo-like kinase-1. Conclusion: Our results indicated that miR-505 suppresses GC cell proliferation and invasion; it may be a valuable candidate gene for seeking therapy strategy for GC. Keywords: MicroRNA-505, EMT, polo-like kinase-1, gastric cancer

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