Uncovering the SUMOylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification

Abstract

Ubiquitylation and SUMOylation are two important related post-translational modifications. Here the authors present an approach for the simultaneous identification and quantification of protein-wide SUMO and ubiquitin sites from a single sample, uncovering widespread crosstalk between the two modifications.