Effect of antibiotics selection on survival rate of nodal explant and gene transformation in Anthurium andraeanum cv. Sonate

Abstract

The effects of various concentrations of hygromycin antibiotic supplemented in modified Murashige and Skoog (MMS) on the survival rate of nodal explant of Anthurium andraeanum cv. Sonate were determined.The use of hygromycin at 50 mg/l caused absolute death of nodal tissue after 4 weeks of culture. Dipping nodal explant with agrobacterium, EHA 105 containing pCAMBIA1301 for 15 min followed by co-cultureon filter paper laid on MMS with 0.5 mg/l benzyladenine (BA), 0.5 mg/l thidiazuron (TDZ) and 200 mg/l cefotaxime for 2 days then transferring the explant to culture on MMS supplemented with the abovephytohormones and 50 mg/l hygromycin resulted in the highest survival rate at 26.6% with 4 shoots/callus. Histochemical analysis of gus activity was found in callus after 6 weeks of nodal culture and in leaf fromshoot derived from the callus.

Keywords

• anthurium
• hygromycin
• node
• gene transformation