First Isolation and Multilocus Sequence Typing of <i>Brucella canis</i> from a Subclinically Infected Pet Dog in China

Guangwen Yan; Zidong Pang; Yan Hu; Ziyao Zhou; Haifeng Liu; Yan Luo; Zhihua Ren; Xiaoping Ma; Suizhong Cao; Liuhong Shen; Ya Wang; Liping Gou; Dongjie Cai; Yanqiu Zhu; Yalin Zhong; Wei Li; Xianpeng Shi; Guangneng Peng; Zhijun Zhong

doi:10.3390/vetsci9010022

Veterinary Sciences (Jan 2022)

First Isolation and Multilocus Sequence Typing of <i>Brucella canis</i> from a Subclinically Infected Pet Dog in China

Guangwen Yan,
Zidong Pang,
Yan Hu,
Ziyao Zhou,
Haifeng Liu,
Yan Luo,
Zhihua Ren,
Xiaoping Ma,
Suizhong Cao,
Liuhong Shen,
Ya Wang,
Liping Gou,
Dongjie Cai,
Yanqiu Zhu,
Yalin Zhong,
Wei Li,
Xianpeng Shi,
Guangneng Peng,
Zhijun Zhong

Affiliations

Guangwen Yan: College of Animal Science, Xichang University, Xichang 615000, China
Zidong Pang: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Yan Hu: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Ziyao Zhou: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Haifeng Liu: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Yan Luo: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Zhihua Ren: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Xiaoping Ma: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Suizhong Cao: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Liuhong Shen: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Ya Wang: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Liping Gou: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Dongjie Cai: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Yanqiu Zhu: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Yalin Zhong: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Wei Li: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Xianpeng Shi: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Guangneng Peng: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Zhijun Zhong: Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

DOI: https://doi.org/10.3390/vetsci9010022
Journal volume & issue: Vol. 9, no. 1
p. 22

Abstract

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Canine brucellosis, a worldwide zoonotic disease, is mainly caused by Brucella canis. In the present study, we isolated a Brucella strain (CD3) from a subclinically infected pet dog in Sichuan Province, Southwestern China. Classical biotyping methods and molecular biological tests (BCSP31 and BcSS PCR) proved that the strain belonged to B. canis. Furthermore, B. canis CD3 and another two B. canis strains (WJ5 and YA4), which were all isolated from pet dogs in Sichuan, were genotyped using multilocus sequence typing (MLST). Our results showed that the three B. canis strains were identified as the same sequence type (ST21). The present study is the first to report B. canis strain from a subclinically infected pet dog in China, indicating a potential threat to public health posed by subclinical infections in pet dogs. We suggest that screening for B. canis should be incorporated into routine medical examination of pet dogs and other companion animals in areas with a history of animal or human brucellosis.

Published in Veterinary Sciences

ISSN: 2306-7381 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture: Animal culture: Veterinary medicine
Website: http://www.mdpi.com/journal/vetsci

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