Are genes of interleukins' 10 (RS1800872) and heat shock protein 70-2 (RS1061581) predictive markers of cytokines production in patients with enterocolitis

Abstract

Objective: to investigate the effect of 1267 AG gene polymorphism of heat shock proteins (HSP) family 70-2 (HSP70-2) and C-592A gene polymorphism of interleukin 10 (IL-10) on the level IL-1β, TNF- and IL-10 in the blood of patients with colienteritis. Materials and methods: 95 patients suffering from Colienteritis participated in the study including 34.74% (33) men, and 65.26% (62) women, their average age was 38.66±3.11 years. Concentration of IL-1β, TNF- та IL-10 in the blood plasma was examined by means of immune-enzyme method. Polymorphism of HSP70-2 (rs1061581) and IL-10 (rs1800872) genes was studied by means of polymerase chain reaction (PCR) method. The control group included 30 practically healthy individuals correlated by their age and sex who were not infected by diseases of any localization during the last 6 months. Results. Changes of immune-inflammatory body response in patients with colienteritis are characterized by an increased synthesis of the first generation pro-inflammatory IL-1β and TNFα on 40.93% and 44.44% (р

Keywords

• colienteritis, coliescherichiosis, gastroenterocolitis, genes hsp70-2, il-10, markers, cytokines il-1β, tnfα, il-10