Crystals (Jun 2023)

3D Printed Microfluidic Cell for SAXS Time-Resolved Measurements of the Structure of Protein Crystallization Solutions

  • Margarita A. Marchenkova,
  • Sergei V. Chapek,
  • Petr V. Konarev,
  • Ksenia B. Ilina,
  • Georgy S. Peters,
  • Yury V. Pisarevsky,
  • Vladimir A. Shishkov,
  • Alexander V. Soldatov,
  • Mikhail V. Kovalchuk

DOI
https://doi.org/10.3390/cryst13060938
Journal volume & issue
Vol. 13, no. 6
p. 938

Abstract

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A multichannel microfluidic cell (MFC) obtained using 3D printing for studying the structure of complex solutions by small-angle X-ray scattering (SAXS) is described. MFC was tested at the BioMUR beamline of the Kurchatov synchrotron. A comparative analysis of SAXS signal from the standard capillary and from the developed MFC was carried out, with MFC showing significant advantages. The dynamics of SAXS scattering curves for lysozyme solutions with NaCl precipitant were studied when the protein and precipitant concentrations changed. The obtained time series of data are well consistent with the known data for the lysozyme solution.

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