Nature Communications (Dec 2020)

Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers

  • Michael J. Petrany,
  • Casey O. Swoboda,
  • Chengyi Sun,
  • Kashish Chetal,
  • Xiaoting Chen,
  • Matthew T. Weirauch,
  • Nathan Salomonis,
  • Douglas P. Millay

DOI
https://doi.org/10.1038/s41467-020-20063-w
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 12

Abstract

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Mammalian skeletal muscle is composed of multinucleated myofibers, containing hundreds of nuclei that coordinate cellular function. Here, the authors show that single-nucleus RNA-sequencing reveals rare and emergent myonuclear populations, and uncovers dynamic transcriptional heterogeneity in development and aging.