Application of cold-adaptive Pseudomonas sp. SDR4 and Mortierella alpina JDR7 co-immobilized on maize cob in remediating PAH-contaminated freeze-thawed soil

Abstract

There are large areas of contaminated soils with low- and medium-concentration of polycyclic aromatic hydrocarbons (PAHs) in the coldest regions of the earth, potentially threatening ecological safety and human health. Using maize cobs (MC) as the carrier, two cold-adaptive PAHs-degrading microorganisms, bacterial (Pseudomonas sp., SDR4) and fungal (Mortierella alpine, JDR7) strains were co-immobilized. The degradation characteristics of phenanthrene (Phe), pyrene (Pyr) and benzo[a]pyrene (BaP) in freeze-thawed soil by both co-immobilized and free microorganism were studied. The removal rate of Phe, Pyr and BaP using the co-immobilized fungal-bacterial consortium within 60 d was 59.2±3.7%, 46.6±3.3%, and 36.8±2.7%, respectively, which was obviously higher than that of free fungal-bacterial consortium in the same time. Ten percent (w/w) was the optimal amount of inoculum for PAH degradation in the co-immobilized fungal-bacterial consortium. Under low-temperature conditions, when the initial concentration of PAH was between 10-100 mg•kg−1, the immobilized cold-adaptive fungal-bacterial consortium displayed the desired degradation of PAHs. The scanning electronic microscope (SEM) observation and mass transmission showed that the microstructure of co-immobilized mixed system was beneficial to the growth of SDR4 and JDR7 at low temperature. These results imply that the cold-adaptive fungal-bacterial consortium, co-immobilized on MC, has the potential for application in remediating PAH contaminated soil under the freeze-thawing environment.

Keywords

• Immobilized microorganisms
• Cold regions
• Organic contaminants
• Remediation
• Soil pollution
• Abbreviations, PAHs, Polycyclic aromatic hydrocarbons