Protocol for high-throughput cloning, expression, purification, and evaluation of bispecific antibodies

Abstract

Summary: Bispecific antibodies are a powerful new class of therapeutics, but their development often requires enormous amounts of time and resources. Here, we describe a high-throughput protocol for cloning, expressing, purifying, and evaluating bispecific antibodies. This protocol enables the rapid screening of large panels of bispecific molecules to identify top candidates for further development.For complete details on the use and execution of this protocol, please refer to Estes et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• High Throughput Screening
• Antibody
• Protein Biochemistry
• Protein expression and purification
• Biotechnology and bioengineering