Cancer Management and Research (May 2020)
The Effect of Hormone Therapy on the Expression of Prostate Cancer and Multi-Epigenetic Marker Genes in a Population of Iranian Patients
Abstract
Mahan Mohammadi,1 Shiva Irani,1 Iman Salahshourifar,1 Jalil Hosseini,2 Afshin Moradi,3 Farkhondeh Pouresmaeili2,4 1Department of Molecular Genetics, Faculty of Science, Science and Research Branch, Islamic Azad University, Tehran, Iran; 2Men’s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 3Department of Pathology, Shahid Beheshti University of Medical Sciences, Shohadaye Tajrish Hospital, Tehran, Iran; 4Medical Genetics Department, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranCorrespondence: Farkhondeh PouresmaeiliMen’s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranTel/ Fax +982 123872572Email [email protected] and Aim: Many recent studies have shown a direct relationship between the decrease in the expression of GSTP1 and RASSF1 with the incidence and progression of prostate cancer. Moreover, the expression level of these genes is greatly affected by epigenetic factors and their methylation pattern. Given the prevalence of prostate cancer and the importance of choosing the best method to inhibit the progression of the disease and provide specific treatment, it is important to evaluate the effect of hormone therapy on the expression of effective prostate cancer genes and epigenetic markers.Patients and Methods: In this case–control study, 35 prostate cancer samples were examined before and after hormone therapy. Following the blood sampling, RNA extraction, and cDNA synthesis, the expression of GSTP1, RASSF1, HDAC, DNMT3A, and DNMT3B was assessed by real-time PCR.Results: The results analysis showed that the expression of GSTP1, RASSF1, and DNMT3B was significantly increased, DNMT3A was significantly decreased (P value< 0.05) and HDAC expression did not change significantly (P value=0.19) after hormone therapy.Discussion: Significant changes in the expression of GSTP1, RASSF1, DNMT3B and DNMT3A in the studied samples indicate that these genes are susceptible targets for cancer hormone therapy in Iranian men like in the other populations. Evaluation of gene activity in a larger population of patients may support these findings.Keywords: GSTP1, RASSF1, HDAC, DNMT3A, DNMT3B, prostate cancer, hormone therapy, real-time PCR
