Comparative Transcriptomic Profiling of Brain and Liver in Phoenix Barbs (<i>Spinibarbus denticulatus denticulatus</i>) with Differential Growth Rates
Xi Xie,
Jiamiao Zhuang,
Xianping Liao,
Zhengsheng Xu,
Wenlang Liang,
Yilin Su,
Li Lin,
Jungang Xie,
Weiqiang Lin
Affiliations
Xi Xie
Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Jiamiao Zhuang
Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Xianping Liao
Fishery Research Institute of Zhaoqing, Zhaoqing 526114, China
Zhengsheng Xu
Fishery Research Institute of Zhaoqing, Zhaoqing 526114, China
Wenlang Liang
Fishery Research Institute of Zhaoqing, Zhaoqing 526114, China
Yilin Su
Fishery Research Institute of Zhaoqing, Zhaoqing 526114, China
Li Lin
Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Science Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Jungang Xie
Fishery Research Institute of Zhaoqing, Zhaoqing 526114, China
Weiqiang Lin
Fishery Research Institute of Zhaoqing, Zhaoqing 526114, China
Phoenix barb (Spinibarbus denticulatus denticulatus) is a notable fish species in South China and is valued for its ecological and economic importance. To elucidate the molecular basis underlying its growth, we collected transcriptome profiles from the brains and livers of individual fish with different growth rates and compared differentially expressed genes (DEGs) at 3 and 9 months after hatching (MAH). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the pathways associated with growth were predominantly enriched in fatty acid biosynthesis, AMPK signaling, PI3K-Akt signaling, estrogen signaling, and protein metabolism. Notably, a greater number of DEGs from the fast-growing group were associated with these pathways at the early growth stage (3 MAH) than at the later stage (9 MAH). Real-time quantitative PCR results further validated that the genes involved in these pathways exhibited higher expression levels in fast-growing samples. This study enhances our understanding of the genetic mechanisms underlying growth rate differences and provides valuable genetic resources for future growth-related molecular breeding programs of phoenix barbs.
