In vitro cytotoxic activity of phytosynthesized silver nanoparticles using Clematis vitalba L. (Ranunculaceae) aqueous decoction
Nicoleta Anca Sutan,
Diana Ionela Popescu (Stegarus),
Oana Alexandra Drăghiceanu,
Carmen Topală,
Claudiu Şuţan,
Aurelian Denis Negrea,
Denisa Ştefania Vîlcoci,
Georgiana Cîrstea,
Sorin Georgian Moga,
Liliana Cristina Soare
Affiliations
Nicoleta Anca Sutan
Department of Natural Sciences, National University of Science and Technology POLITEHNICA Bucharest, Pitesti University Centre
Diana Ionela Popescu (Stegarus)
National Research and Development Institute for Cryogenics and Isotopic Technologies–ICSI Ramnicu Valcea, 4th Uzinei Street, 240050 Ramnicu Valcea
Oana Alexandra Drăghiceanu
Department of Natural Sciences, National University of Science and Technology POLITEHNICA Bucharest, Pitesti University Centre
Carmen Topală
Department of Environmental Engineering and Applied Sciences, National University of Science and Technology POLITEHNICA Bucharest, Pitesti University Centre
Claudiu Şuţan
Department of Environmental Engineering and Applied Sciences, National University of Science and Technology POLITEHNICA Bucharest, Pitesti University Centre
Aurelian Denis Negrea
Regional Center of Research and Development for Materials, Processes and Innovative Products Dedicated to the Automotive Industry, National University of Science and Technology POLITEHNICA Bucharest, Pitesti University Centre
Denisa Ştefania Vîlcoci
Regional Center of Research and Development for Materials, Processes and Innovative Products Dedicated to the Automotive Industry, National University of Science and Technology POLITEHNICA Bucharest, Pitesti University Centre
Georgiana Cîrstea
Regional Center of Research and Development for Materials, Processes and Innovative Products Dedicated to the Automotive Industry, National University of Science and Technology POLITEHNICA Bucharest, Pitesti University Centre
Sorin Georgian Moga
Regional Center of Research and Development for Materials, Processes and Innovative Products Dedicated to the Automotive Industry, National University of Science and Technology POLITEHNICA Bucharest, Pitesti University Centre
Liliana Cristina Soare
Department of Natural Sciences, National University of Science and Technology POLITEHNICA Bucharest, Pitesti University Centre
In this study, we report a bottom-up approach for silver nanoparticles (AgNPs) synthesis using aqueous decoction of aerial parts of Clematis vitalba L. The phytosynthesized AgNPs were characterized by X-ray diffraction (XRD), UV-vis spectroscopy, Fourier Transform-Infrared Spectroscopy (FTIR), Scanning Electron Microscopy coupled with Energy Dispersive X-ray Spectroscopy (SEM-EDS) and Bright Field Scanning Transmission Electron Microscopy (BFSTEM). The cytogenotoxicity and phytotoxicity assays of AgNPs were assessed by using Allium test, Evans blue and 2, 3, 5-triphenyl tetrazolium chloride (TTC) staining, root and stem growth potential, and biomass evaluation. The results revealed that AgNPs were in the size range of 1-15 nm and spherical shape. The biosynthesized AgNPs augment the mitodepressive effect, disruption of cellular metabolism, impairment of root and stem growth, and biomass reduction induced by C. vitalba aqueous extracts. These results outline the toxicological profile of the C. vitalba extracts, as well as of the phytogenerated AgNPs and provides scientific perspectives on the use of C. vitalba extracts as reducing and stabilizing agent for the phytosynthesis of metallic nanoparticles.
