Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing

Thomas A. Hamilton; Gregory M. Pellegrino; Jasmine A. Therrien; Dalton T. Ham; Peter C. Bartlett; Bogumil J. Karas; Gregory B. Gloor; David R. Edgell

doi:10.1038/s41467-019-12448-3

Nature Communications (Oct 2019)

Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing

Thomas A. Hamilton,
Gregory M. Pellegrino,
Jasmine A. Therrien,
Dalton T. Ham,
Peter C. Bartlett,
Bogumil J. Karas,
Gregory B. Gloor,
David R. Edgell

Affiliations

Thomas A. Hamilton: Department of Biochemistry, Schulich School of Medicine and Dentistry
Gregory M. Pellegrino: Department of Biochemistry, Schulich School of Medicine and Dentistry
Jasmine A. Therrien: Department of Biochemistry, Schulich School of Medicine and Dentistry
Dalton T. Ham: Department of Biochemistry, Schulich School of Medicine and Dentistry
Peter C. Bartlett: Department of Biochemistry, Schulich School of Medicine and Dentistry
Bogumil J. Karas: Department of Biochemistry, Schulich School of Medicine and Dentistry
Gregory B. Gloor: Department of Biochemistry, Schulich School of Medicine and Dentistry
David R. Edgell: Department of Biochemistry, Schulich School of Medicine and Dentistry

DOI: https://doi.org/10.1038/s41467-019-12448-3
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 9

Abstract

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CRISPR nucleases can be programmed to cleave sequences in specific bacteria to induce cell death. Here, Hamilton et al. present an optimized method for conjugative delivery of CRISPR nucleases, consisting of a single plasmid that encodes both the conjugative machinery and the nuclease.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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