Host Specificity and Differential Pathogenicity of <i>Pectobacterium</i> Strains from Dicot and Monocot Hosts

Nirmal Khadka; Janak Raj Joshi; Noam Reznik; Nofar Chriker; Adi Nudel; Einat Zelinger; Zohar Kerem; Iris Yedidia

doi:10.3390/microorganisms8101479

Microorganisms (Sep 2020)

Host Specificity and Differential Pathogenicity of <i>Pectobacterium</i> Strains from Dicot and Monocot Hosts

Nirmal Khadka,
Janak Raj Joshi,
Noam Reznik,
Nofar Chriker,
Adi Nudel,
Einat Zelinger,
Zohar Kerem,
Iris Yedidia

Affiliations

Nirmal Khadka: Department of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, 7610001 Rehovot, Israel
Janak Raj Joshi: Department of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, 7610001 Rehovot, Israel
Noam Reznik: Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, POB 15159, 752880 Rishon Lezion, Israel
Nofar Chriker: Department of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, 7610001 Rehovot, Israel
Adi Nudel: Department of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, 7610001 Rehovot, Israel
Einat Zelinger: Department of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, 7610001 Rehovot, Israel
Zohar Kerem: Department of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, 7610001 Rehovot, Israel
Iris Yedidia: Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, POB 15159, 752880 Rishon Lezion, Israel

DOI: https://doi.org/10.3390/microorganisms8101479
Journal volume & issue: Vol. 8, no. 10
p. 1479

Abstract

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Recent phylogenetic studies have transferred certain isolates from monocot plants previously included in the heterogeneous group of Pectobacteriumcarotovorum (Pc) to a species level termed Pectobacterium aroidearum. The specificity of Pectobacterium associated infections had received less attention, and may be of high scientific and economic importance. Here, we have characterized differential responses of Pectobacterium isolates from potato (WPP14) and calla lily (PC16) on two typical hosts: Brassica oleracea var. capitata (cabbage) a dicot host; and Zantedeschia aethiopica (calla lily) a monocot host. The results revealed clear host specific responses following infection with the two bacterial strains. This was demonstrated by differential production of volatile organic compounds (VOCs) and the expression of plant defense-related genes (pal, PR-1, lox2, ast). A related pattern was observed in bacterial responses to each of the host’s extract, with differential expression of virulence-related determinants and genes associated with quorum-sensing and plant cell wall-degrading enzymes. The differences were associated with each strain’s competence on its respective host.

Published in Microorganisms

ISSN: 2076-2607 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.mdpi.com/journal/microorganisms

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