Proportion-dependent osteogenic activity of electrospun nano-hydroxyapatite/polylactic acid fiber membrane in vitro and in vivo

Bangbang Liang; Tianyi Feng; Xiaoting Yuan; Kang Zhao; Chengyu Li; Yingchao Han

Materials & Design (Jul 2022)

Proportion-dependent osteogenic activity of electrospun nano-hydroxyapatite/polylactic acid fiber membrane in vitro and in vivo

Bangbang Liang,
Tianyi Feng,
Xiaoting Yuan,
Kang Zhao,
Chengyu Li,
Yingchao Han

Affiliations

Bangbang Liang: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, PR China
Tianyi Feng: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, PR China
Xiaoting Yuan: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, PR China
Kang Zhao: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, PR China
Chengyu Li: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, PR China
Yingchao Han: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, PR China; Foshan Xianhu Laboratory of the Advanced Energy Science and Technology Guangdong Laboratory, Xianhu Hydrogen Valley, Foshan 528200, PR China; Corresponding author at: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, PR China.

Journal volume & issue: Vol. 219
p. 110834

Abstract

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The effect of proportion on the physicochemical and osteogenic properties of electrospun nano-hydroxyapatite/polylactic acid (nHAP/PLA) fiber membrane was investigated from 0/100 to 20/80 (nHAP/PLA mass ratio), aiming to optimise the proportion and integrate mechanical support, biodegradability, cell infiltration and osteogenic activity for better bone repair. The results showed the incorporated nHAP increased the hydrophilicity of the fiber surface, and 6–10 wt% nHAP/PLA membrane showed the enhanced modulus in the direction perpendicular to membrane plane. Biological experiments demonstrated the nHAP proportion-dependent osteogenic activity of nHAP/PLA membranes. The addition of 6–10 wt% nHAP in the fiber membrane significantly promoted cell proliferation and differentiation. In vivo experiments of stacked implantation of the membrane displayed multilayer growth in the pure PLA group owing to the hydrophobicity of PLA and single-layer growth in the nHAP/PLA group because the hydrophilicity of the nHAP/PLA membrane improved the adhesion of the layers. The addition of 6–10 wt% nHAP/PLA also promoted cell recruitment and infiltration and increased osteogenesis, with enhancements of 41% in bone tissue volume/total tissue volume and 37% in bone mineral density. Our study provides helpful support for the preparation of composite electrospun membranes with improved osteogenic activity by optimising the organic–inorganic proportion.

Published in Materials & Design

ISSN: 0264-1275 (Print); 1873-4197 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.journals.elsevier.com/materials-and-design

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