Protocol for measuring NLRC4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages

Xingchen Dong; Lin-Feng Chen

STAR Protocols (Dec 2021)

Protocol for measuring NLRC4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages

Xingchen Dong,
Lin-Feng Chen

Affiliations

Xingchen Dong: Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Corresponding author
Lin-Feng Chen: Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 4
p. 100894

Abstract

Read online

Summary: NLR family CARD domain containing protein 4 (NLRC4) inflammasome activation and the associated pyroptosis are critical for protection against infection by bacterial pathogens. This protocol presents a detailed procedure to activate and measure NLRC4 inflammasome activation and pyroptosis upon Salmonella Typhimurium infection. The techniques can be adapted to monitoring the activation of other types of inflammasomes and pathogenic stimuli.For comprehensive details on the use and execution of this protocol, please refer to Dong et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

About the journal

Abstract

Keywords