Genetic Diversity and Possible Origins of the Hepatitis B Virus in Siberian Natives
Victor Manuylov,
Vladimir Chulanov,
Ludmila Bezuglova,
Elena Chub,
Anastasia Karlsen,
Karen Kyuregyan,
Yulia Ostankova,
Alexander Semenov,
Ludmila Osipova,
Tatjana Tallo,
Irina Netesova,
Artem Tkachuk,
Vladimir Gushchin,
Sergey Netesov,
Lars O. Magnius,
Heléne Norder
Affiliations
Victor Manuylov
Gamaleya National Research Center of Epidemiology and Microbiology, 123098 Moscow, Russia
Vladimir Chulanov
National Medical Research Center for Phthisiopulmonology and Infectious Diseases, 127473 Moscow, Russia
Ludmila Bezuglova
Hepatitis B ELISA Department, Vector-Best JSC, 630559 Koltsovo, Russia
Elena Chub
Department of Molecular Virology of Flaviviruses and Viral Hepatitis, State Research Center of Virology and Biotechnology “Vector” of the Rospotrednadzor, 630559 Koltsovo, Russia
Anastasia Karlsen
Department of Viral Hepatitis, Russian Medical Academy of Continuous Professional Education, 125993 Moscow, Russia
Karen Kyuregyan
Department of Viral Hepatitis, Russian Medical Academy of Continuous Professional Education, 125993 Moscow, Russia
Yulia Ostankova
Laboratory of Molecular Immunology, Institute Pasteur in Saint Petersburg for Research in Epidemiology and Microbiology of the Rospotrednadzor, 197101 Saint-Petersburg, Russia
Alexander Semenov
Ekaterinburg Research Institute of Viral Infections of SRC VB Vector, 620030 Ekaterinburg, Russia
Ludmila Osipova
Laboratory of Populational Ethnogenetics, Department of Molecular Diagnostics and Epidemiology, Institute of Cytology and Genetics, 630090 Novosibirsk, Russia
Tatjana Tallo
Department of Microbiology, Public Health Agency of Sweden, 171 82 Stockholm, Sweden
Irina Netesova
Hepatitis B ELISA Department, Vector-Best JSC, 630559 Koltsovo, Russia
Artem Tkachuk
Gamaleya National Research Center of Epidemiology and Microbiology, 123098 Moscow, Russia
Vladimir Gushchin
Gamaleya National Research Center of Epidemiology and Microbiology, 123098 Moscow, Russia
Sergey Netesov
Laboratory of Bionanotechnology, Microbiology and Virology, Novosibirsk State University, 630090 Novosibirsk, Russia
Lars O. Magnius
Ulf Lundahl’s Foundation, 100 61 Stockholm, Sweden
Heléne Norder
Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg, 413 90 Gothenburg, Sweden
A total of 381 hepatitis B virus (HBV) DNA sequences collected from nine groups of Siberian native populations were phylogenetically analyzed along with 179 HBV strains sampled in different urban populations of former western USSR republics and 50 strains from Central Asian republics and Mongolia. Different HBV subgenotypes predominated in various native Siberian populations. Subgenotype D1 was dominant in Altaian Kazakhs (100%), Tuvans (100%), and Teleuts (100%) of southern Siberia as well as in Dolgans and Nganasans (69%), who inhabit the polar Taimyr Peninsula. D2 was the most prevalent subgenotype in the combined group of Nenets, Komi, and Khants of the northern Yamalo-Nenets Autonomous Region (71%) and in Yakuts (36%) from northeastern Siberia. D3 was the main subgenotype in South Altaians (76%) and Buryats (40%) of southeastern Siberia, and in Chukchi (51%) of the Russian Far East. Subgenotype C2 was found in Taimyr (19%) and Chukchi (27%), while subgenotype A2 was common in Yakuts (33%). In contrast, D2 was dominant (56%) in urban populations of the former western USSR, and D1 (62%) in Central Asian republics and Mongolia. Statistical analysis demonstrated that the studied groups are epidemiologically isolated from each other and might have contracted HBV from different sources during the settlement of Siberia.
