Journal of Forensic Science and Medicine (Jan 2018)

The application of next-generation sequencing to validate D12S391 microvariation

  • Qingxia Zhang,
  • Jinjie Liu,
  • He Ren,
  • Shuai Sun,
  • Yi Zhao,
  • Chong Chen,
  • Li Jia,
  • Yacheng Liu,
  • Jiangwei Yan

DOI
https://doi.org/10.4103/jfsm.jfsm_62_17
Journal volume & issue
Vol. 4, no. 2
pp. 67 – 69

Abstract

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Objective: In a paternity case, the D12S391 locus was reported as a mismatch. To confirm the existence of mutations and mutations come from father or mother. Methods: STR and next-generation sequencing technology were used to validate the sequence. Results: NGS showed the loss of one adenine between the 19.3 allele of the child and allele 20 of the mother. Conclusion: The NGS can be applied in the paternity to validate the mutation.

Keywords