Majallah-i ̒Ilmī-i Shīlāt-i Īrān (Jan 2019)
Effect of dietary EDTA supplementation on reduction of heavy metals load in common carp (Cyprinus carpio) muscle
Abstract
In this study the effects of various amounts of dietary EDTA (ethylene di amine tetra-acetic acid) supplementation on the amounts of cadmium, mercury, lead and arsenic were evaluated over a growing period of 90 days. This research was conducted according to completely randomized design with 5 treatments including 1(the control), 2, 3, 4 and 5 containing different EDTA concentration of 75, 150, 300 and 600 ppm, respectively and 6 replicates. The average concentration of arsenic ranged from total weight, total length and body height in fingerling fishes were 2.1±0.06 g, 1.96±0.05 and 0.51±0.01 cm, respectively. The average concentration of arsenic ranged from 56 to 84.66 µg/kg in different treatments. The highest and lowest concentration of arsenic were observed in treatment 2 and 5 were significantly different among various treatments (P>0.05).The highest and the lowest concentration of mercury were observed in treatment 3 (129.33±2.4 µg/kg) and 4 (112.±332.02 µg/kg), respectively. The amounts of mercury in treatment 1, 4 and 5 were significantly different from treatments 2 and 3(P<0.05). Lead concentration ranged from 192 to 256 µg/kg, in which the treatment 4 showed a significantly different as compared to the other (P<0.05). The lowest and the highest concentration of cadmium were observed in treatments 2 (106.00±4.72 µg/kg) and 4 (113.0±4.61 µg/kg), respectively. The concentration of cadmium in treatment4 was a significantly different from treatments 1, 2 and5 (P<0.05).
