Nature Communications (Feb 2017)

Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo

  • Taro Ozaki,
  • Kona Yamashita,
  • Yuki Goto,
  • Morito Shimomura,
  • Shohei Hayashi,
  • Shumpei Asamizu,
  • Yoshinori Sugai,
  • Haruo Ikeda,
  • Hiroaki Suga,
  • Hiroyasu Onaka

DOI
https://doi.org/10.1038/ncomms14207
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 13

Abstract

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Goadsporin is an antibacterial peptide highly customized by multiple post-translational modifying enzymes. Here, the authors dissect its biosynthetic pathway byin vitro reconstitution and use their insights to produce goadsporin analogues in vitro and in vivo.