二乙烯三胺脱除花生粕中黄曲霉毒素B1的 工艺优化及机制研究Process optimization and mechanism research for removal of aflatoxin B1 from peanut meal using diethylenetriamine

Abstract

旨在为霉变花生粕中黄曲霉毒素B1（AFB1）的脱除提供技术支持，以自然霉变花生粕为实验材料，用二乙烯三胺脱除花生粕中的AFB1。采用单因素实验优化脱毒条件，并对二乙烯三胺脱除AFB1的机制进行研究。结果表明：二乙烯三胺脱除花生粕中AFB1的最佳工艺条件为液料比5∶ 1、处理温度50 ℃、处理时间45 min、二乙烯三胺溶液质量浓度10 mg/mL，在此条件下AFB1脱除率为95.5%；高效液相色谱和高分辨质谱分析表明，AFB1可能的降解机制是其酮羰基与二乙烯三胺发生脱水反应，并且反应产物通过离心随着上清液被脱除。二乙烯三胺具有应用于花生粕中AFB1脱除的潜力。To provide a technical support for the removal of aflatoxin B1 (AFB1) from moldy peanut meal, with naturally moldy peanut meal as the experimental material, and diethylenetriamine was used to the removal of AFB1.Single factor experiment was used to optimize the detoxification conditions, and the mechanism of removing AFB1 by diethylenetriamine was studied. The results showed that the optimal conditions for the removal of AFB1 from peanut meal were obtained as follows: liquid-solid ratio 5∶ 1, reaction temperature 50 ℃, reaction time 45 min, mass concentration of diethylenetriamine 10 mg/mL. Under these conditions, the detoxification rate was 95.5%. The high performance liquid chromatography and high-resolution mass spectrometry analysis showed that the possible degradation way of AFB1 was the dehydration reaction between the ketone carbonyl group in AFB1 and diethylenetriamine. In addition, in the process of detoxification, reaction products were removed by centrifugation with supernatant. In conclusion, diethylenetriamine has the potential to be used for the removal of AFB1 from peanut meal.

Keywords

• 花生粕；黄曲霉毒素b1；二乙烯三胺; 脱毒机制
• peanut meal; aflatoxin b1; diethylenetriamine; detoxification mechanism