Zhongguo youzhi (Apr 2022)
干燥方式对迷迭香脂溶性抗氧化物质 得率及抗氧化活性的影响 Effects of drying methods on yield and antioxidant activity of fat-soluble antioxidant components from Rosmarinus officinalis
Abstract
通过研究不同生长期对迷迭香脂溶性抗氧化物质（鼠尾草酸、鼠尾草酚和熊果酸）得率的影响筛选出最佳生长期，在此基础上测定不同干燥方式（阴干、烘干（45 ℃）和晾晒）下迷迭香脂溶性抗氧化物质得率，DPPH自由基、ABTS+自由基、羟自由基清除能力和铁还原力，以考察不同干燥方式对迷迭香脂溶性抗氧化物质抗氧化活性的影响。结果表明：迷迭香抗氧化物质积累的最佳生长期为10个月，3种不同干燥方式中烘干处理的迷迭香所提脂溶性抗氧化物质的得率最大，DPPH自由基、ABTS+自由基、羟自由基清除能力和铁还原力均最强，但弱于TBHQ，其清除DPPH自由基、ABTS+自由基、羟自由基的半抑制浓度（IC50）分别为0.034、0.068、0.067 mg/mL。因此，采用生长期10个月、烘干处理的迷迭香所提脂溶性抗氧化物质具有较强的抗氧化活性，能较好地保留迷迭香脂溶性抗氧化物质，适用于工业生产。The optimal growth period was selected by studying the effect of different growth periods on the yield of fat-soluble antioxidant components (carnosic acid, carnosol and ursolic acid) in Rosmarinus officinalis，then the yield of Rosmarinus officinalis fat-soluble antioxidant components, the scavenging capacity of DPPH radical, ABTS+ radical, hydroxyl radical and iron reducing power were determined under different drying methods (shade drying, sun drying and hot air drying at 45 ℃) to investigate the effect of different drying methods on the antioxidant activity of Rosmarinus officinalis fat-soluble antioxidant components.The results showed that ten months was the optimal growth period for the accumulation of fat-soluble antioxidant components in Rosmarinus officinalis.Rosmarinus officinalis treated with hot air drying had the highest yield of fat-soluble antioxidant components, the strongest scavenging ability of DPPH radical, ABTS+ radical, hydroxyl radical and iron reducing power, but weaker than TBHQ, and its semi-inhibitory concentration(IC50) of scavenging DPPH radical, ABTS+ radical and hydroxyl radical were 0.034,0.068, 0.067 mg/mL,respectively.In general,the hot air-dried Rosmarinus officinalis grew for ten months showed stronger antioxidant activity,which could well retain the fat-soluble antioxidant components of Rosmarinus officinalis,and it was suitable for industrial production.
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