OncoTargets and Therapy (Apr 2020)

Long Non-Coding RNA HAND2-AS1 Inhibits Growth and Migration of Gastric Cancer Cells Through Regulating the miR-590-3p/KCNT2 Axis

  • Yu L,
  • Li H,
  • Li Z,
  • Jia J,
  • Wu Z,
  • Wang M,
  • Li F,
  • Feng Z,
  • Xia H,
  • Gao G

Journal volume & issue
Vol. Volume 13
pp. 3187 – 3196

Abstract

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Lan Yu,1 Hui Li,2 Zhiying Li,1 Jianchao Jia,1 Zhouying Wu,1 Min Wang,1 Feng Li,1 Zongqi Feng,1 Huilin Xia,3 Guanxin Gao3 1Clinical Medical Research Center, Inner Mongolia People’s Hospital, Hohhot 010017, People’s Republic of China; 2Department of Oncology, Inner Mongolia People’s Hospital, Hohhot 010017, People’s Republic of China; 3Medical Engineering Department, Inner Mongolia People’s Hospital, Hohhot 010017, People’s Republic of ChinaCorrespondence: Huilin Xia; Guanxin GaoMedical Engineering Department, Inner Mongolia People’s Hospital, No. 20 Zhaowuda Road, Saihan District, Hohhot 010017, People’s Republic of ChinaEmail [email protected]; [email protected]: Long non-coding RNAs (lncRNAs) are regarded as crucial regulators for cancer initiation and progression. Heart and Neural Crest Derivatives Expressed 2 antisense RNA 1 (HAND2-AS1) was recently proposed to function as tumor suppressor in several human cancers. However, its role in gastric cancer (GC) remains unclear.Methods: HAND2-AS1 expression in GC tissues and normal tissues was analyzed at GEPIA (a web server for gene expression profiling analysis). Moreover, RT-qPCR method was utilized to explore HAND2-AS1 expression in GC cells and normal cell. In vitro experiments were carried out using cell counting kit-8 assay, colony formation assay, and flow cytometry assay, respectively. Bioinformatic analysis and luciferase activity reporter assay were performed to identify the downstream targets of HAND2-AS1.Results: We found HAND2-AS1 has decreased expression in both GC tissues and cells. Overexpression of HAND2-AS1 was able to inhibit GC cell proliferation, colony formation, but promote apoptosis. On the contrary, knockdown of HAND2-AS1 could cause the opposite effects on GC cells. Furthermore, HAND2-AS1 was shown to function as a competitive RNA that binds with microRNA-590-3p (miR-590-3p) to affect the expression of potassium sodium-activated channel subfamily T member 2 (KCNT2).Discussion: Our results indicated the tumor suppressive role of HAND2-AS1 in GC. Also, the newly identified HAND2-AS1/miR-590-3p/KCNT2 axis will help us to understand the role of HAND2-AS1 in cancer.Keywords: HAND2-AS1, miR-590-3p, KCNT2, gastric cancer

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