miR319-Regulated TCP3 Modulates Silique Development Associated with Seed Shattering in Brassicaceae
Biting Cao,
Hongfeng Wang,
Jinjuan Bai,
Xuan Wang,
Xiaorong Li,
Yanfeng Zhang,
Suxin Yang,
Yuke He,
Xiang Yu
Affiliations
Biting Cao
School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Hongfeng Wang
National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Science, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Fenglin Road 300, Shanghai 200032, China
Jinjuan Bai
National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Science, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Fenglin Road 300, Shanghai 200032, China
Xuan Wang
National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Science, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Fenglin Road 300, Shanghai 200032, China
Xiaorong Li
National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Science, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Fenglin Road 300, Shanghai 200032, China
Yanfeng Zhang
Hybrid Rape Research Center of Shaanxi Province, Yangling 712100, China
Suxin Yang
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Changchun 130102, China
Yuke He
National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Science, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Fenglin Road 300, Shanghai 200032, China
Xiang Yu
School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Seed shattering is an undesirable trait that leads to crop yield loss. Improving silique resistance to shattering is critical for grain and oil crops. In this study, we found that miR319-targeted TEOSINTE BRANCHED 1, CYCLOIDEA, and PROLIFERATING CELL NUCLEAR ANTIGEN BINDING FACTOR (TCPs) inhibited the process of post-fertilized fruits (silique) elongation and dehiscence via regulation of FRUITFULL (FUL) expression in Arabidopsis thaliana and Brassica napus. AtMIR319a activation resulted in a longer silique with thickened and lignified replum, whereas overexpression of an miR319a-resistant version of AtTCP3 (mTCP3) led to a short silique with narrow and less lignified replum. Further genetic and expressional analysis suggested that FUL acted downstream of TCP3 to negatively regulate silique development. Moreover, hyper-activation of BnTCP3.A8, a B. napus homolog of AtTCP3, in rapeseed resulted in an enhanced silique resistance to shattering due to attenuated replum development. Taken together, our findings advance our knowledge of TCP-regulated silique development and provide a potential target for genetic manipulation to reduce silique shattering in Brassica crops.
