In silico Analysis Predicts that Mir-6770-5p Can Target the X Gene of All Hepatitis B Virus Genotypes

Abstract

To date, effective medication against hepatitis B virus (HBV) has not been developed. MicroRNAs (miRNAs) comprise a promising therapeutic approach to inhibit the virus. In this study, 1917 miRNAs in the miRBase database were screened using bioinformatics software to obtain candidates that can target HBV genotype B. Two parameters, namely pairing pattern and minimum free energy were used to select the qualifying miRNAs. Three miRNAs targeting the X gene and one miRNA targeting the C gene were identified out of 39 initial candidates. Uniquely, miR-6770-5p was the only candidate that could target the X gene of all HBV genotypes, with a higher potency of inhibition compared to other candidates. The three other candidates also showed good potency for some genotypes; thus, the identified candidates show promise as therapeutics for hepatitis infection.

Keywords

• human mirnas
• hbv
• x gene
• in silico analysis