Possibility of Using Modified Polyamide Membranes for Virus Concentration for the Purposes of Sanitary-Virological Analysis of Water

Abstract

The results of investigations of modified polyamide microfiltration membranes application in the concentration of viruses and bacteriophages during sanitary virological monitoring of water bodies are presented in this paper. The analysis of developments in the control of viral contamination of different origins water bodies is given. Viruses presented in the water in small amounts can cause disease. It is shown that an important step is viral concentration from large amounts of water for its identification. The possibility of positively charged microfiltration membranes application in the concentration of viruses is established. A method of polyamide microfiltration membranes modification for increasing the positive charge on their surface is developed. To estimate the retention capacity of membranes the methods of measuring the sorption properties of the membranes using the model dye bromophenyl blue is developed. A membrane filter module with tangentially-radial mode of fluid movement was used. Studies carried out in specialized organizations revealed that the efficiency of bacteriophage MS-2 sorption on the modified polyamide membrane at different loads was 100%. It was found that the use of membrane filtration module not only improves the detention of microorganisms, but also allows improving the elution stage. The laboratory results were confirmed by tests using natural waters.