In April 1947 it was discovered through an experimental animal model a jungle fever. During the experiment, monkeys (macaques) were placed in cages and left exposed in the Zika forest (Uganda); one of the monkeys became ill and died. They filtered postmortem monkey tissues were inoculated into mouse brain and from there, a virus called Zika (ZIKV) was cultured. In 1948 ZIKV was also isolated from mosquitoes Aedes africanus captured in the Zika forest. In 1956 ZIKV transmission in mosquitoes Ae. aegypti was found, as well as a monkey. Between 1968 and 1975, ZIKV was subsequently isolated from humans (Nigeria); 40% of those patients analized by PRNT shown antibodies.