<em>In vitro</em> response of potato plants against the culture filtrate of <em>Alternaria solani</em> Sor. obtained in different culture media

Abstract

This work was developed in order to determine the in vitro response of potato plants against the culture filtrate of Alternaria solani Sor. obtained in two culture media. To obtain filtrate culture were used CCIBP-VAs4 isolate and Fries and Richard culture media. The phytotoxicity of the filtrates was evaluated on in vitro plants susceptible (cv. ‘Desirée’) and resistant (the specie S. chacoense type Pi 275136) by immersion the roots of plants. The results showed that the filtrates obtained in both culture media were phytotoxic on both plant materials. However, culture filtrates that produced in Richard culture medium gave a better differentiation between potato plants cv. ‘Desiree’ (susceptible) and those of the wild species Solanum chacoense PI 275136 (resistant). This result was corresponded with the higher growth of the fungus in this culture medium. Keywords: in vitro differentiation, Fries and Richard culture medium, Solanum tuberosum L