A late-stage assembly checkpoint of the human mitochondrial ribosome large subunit

Pedro Rebelo-Guiomar; Simone Pellegrino; Kyle C. Dent; Aldema Sas-Chen; Leonor Miller-Fleming; Caterina Garone; Lindsey Van Haute; Jack F. Rogan; Adam Dinan; Andrew E. Firth; Byron Andrews; Alexander J. Whitworth; Schraga Schwartz; Alan J. Warren; Michal Minczuk

doi:10.1038/s41467-022-28503-5

Nature Communications (Feb 2022)

A late-stage assembly checkpoint of the human mitochondrial ribosome large subunit

Pedro Rebelo-Guiomar,
Simone Pellegrino,
Kyle C. Dent,
Aldema Sas-Chen,
Leonor Miller-Fleming,
Caterina Garone,
Lindsey Van Haute,
Jack F. Rogan,
Adam Dinan,
Andrew E. Firth,
Byron Andrews,
Alexander J. Whitworth,
Schraga Schwartz,
Alan J. Warren,
Michal Minczuk

Affiliations

Pedro Rebelo-Guiomar: MRC Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus
Simone Pellegrino: Cambridge Institute for Medical Research, University of Cambridge, Cambridge Biomedical Campus
Kyle C. Dent: Cambridge Institute for Medical Research, University of Cambridge, Cambridge Biomedical Campus
Aldema Sas-Chen: Department of Molecular Genetics, Weizmann Institute of Science
Leonor Miller-Fleming: MRC Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus
Caterina Garone: MRC Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus
Lindsey Van Haute: MRC Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus
Jack F. Rogan: STORM Therapeutics Limited, Babraham Research Campus
Adam Dinan: Department of Pathology, University of Cambridge
Andrew E. Firth: Department of Pathology, University of Cambridge
Byron Andrews: STORM Therapeutics Limited, Babraham Research Campus
Alexander J. Whitworth: MRC Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus
Schraga Schwartz: Department of Molecular Genetics, Weizmann Institute of Science
Alan J. Warren: Cambridge Institute for Medical Research, University of Cambridge, Cambridge Biomedical Campus
Michal Minczuk: MRC Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus

DOI: https://doi.org/10.1038/s41467-022-28503-5
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

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Rebelo-Guiomar et al. unveil late stage assembly intermediates of the human mitochondrial ribosome by inactivating the methyltransferase MRM2 in cells. Absence of MRM2 impairs organismal homeostasis, while its catalytic activity is dispensable for mitoribosomal biogenesis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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