Optimization of Glutathione Adhesion Process to Modified Graphene Surfaces
Witold Jakubowski,
Radomir Atraszkiewicz,
Dorota Nowak,
Damian Batory,
Witold Szymański,
Anna Sobczyk-Guzenda,
Łukasz Kaczmarek,
Piotr Kula,
Marian Cłapa,
Tomasz Warga,
Małgorzata Czerniak-Reczulska
Affiliations
Witold Jakubowski
Division of Biophysics, Institute of Materials Science and Engineering, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland
Radomir Atraszkiewicz
Division of Surface Engineering and Heat Treatment, Institute of Materials Science and Engineering, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland
Dorota Nowak
Division of Biomedical Engineering and Functional Materials, Institute of Materials Science and Engineering, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland
Damian Batory
Department of Vehicles and Fundamentals of Machine Design, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland
Witold Szymański
Division of Nanomaterials Engineering, Institute of Materials Science and Engineering, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland
Anna Sobczyk-Guzenda
Division of Coating, Polymer and Non-Metal Engineering, Institute of Materials Science and Engineering, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland
Łukasz Kaczmarek
Division of Advanced Materials and Composite, Institute of Materials Science and Engineering, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland
Piotr Kula
Division of Surface Engineering and Heat Treatment, Institute of Materials Science and Engineering, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland
Marian Cłapa
Division of Biomedical Engineering and Functional Materials, Institute of Materials Science and Engineering, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland
Tomasz Warga
Division of Advanced Materials and Composite, Institute of Materials Science and Engineering, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland
Małgorzata Czerniak-Reczulska
Division of Biomedical Engineering and Functional Materials, Institute of Materials Science and Engineering, Lodz University of Technology, 1/15 Stefanowskiego St., 90-924 Lodz, Poland
The presented work shows the results of the functionalization of the graphene surface obtained by the growth on the liquid bimetallic matrices method. We used glutathione (GSH) as a peptide model, which allowed us to optimize the procedure to obtain high process efficiency. To establish the amount of GSH attached to the graphene surface, the Folina-Ciocalteu method was used, which allows the assessment of the concentration of colored reaction products with peptide bonds without the disadvantages of most methods based on direct colored reaction of peptide bonds. Samples surface morphology, quality of graphene and chemical structure in the subsequent stages of surface modification were tested—for this purpose Raman spectroscopy, scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FTIR) were used.
