Identification of octyl gallate, a novel apoptosis-inducing compound for colon cancer therapy, from Sanguisorba officinalis L. by cell membrane chromatography and UHPLC-(Q)TOF-MS/MS
Chengyang Ni,
Liang Yue,
Mei Ran,
Long Wang,
Feihong Huang,
Shuo Yang,
Jia Lai,
Nan Jiang,
Xinwu Huang,
Dalian Qin,
Hua Li,
Jie Zhou,
Jing Zeng,
Anguo Wu,
Jianming Wu
Affiliations
Chengyang Ni
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China
Liang Yue
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China; Department of Pharmacy, Deyang People's Hospital, Deyang, 618000, China
Mei Ran
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China; School of Basic Medical Sciences, Southwest Medical University, Luzhou, 646000, China
Long Wang
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China
Feihong Huang
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China
Shuo Yang
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China
Jia Lai
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China
Nan Jiang
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China
Xinwu Huang
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China
Dalian Qin
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China; Education Ministry Key Laboratory of Medical Electrophysiology, Sichuan Key Medical Laboratory of New Drug Discovery and Druggability Evaluation, Luzhou Key Laboratory of Activity Screening and Druggability Evaluation for Chinese Materia Medica, Southwest Medical University, Luzhou, Sichuan, 646000, China
Hua Li
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China
Jie Zhou
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China
Jing Zeng
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China; Corresponding author.
Anguo Wu
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China; Corresponding author.
Jianming Wu
School of Basic Medical Sciences, Southwest Medical University, Luzhou, 646000, China; Education Ministry Key Laboratory of Medical Electrophysiology, Sichuan Key Medical Laboratory of New Drug Discovery and Druggability Evaluation, Luzhou Key Laboratory of Activity Screening and Druggability Evaluation for Chinese Materia Medica, Southwest Medical University, Luzhou, Sichuan, 646000, China; Corresponding author. School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.
Colon cancer is a common gastrointestinal malignancy that ranks third in incidence among gastrointestinal cancers. Therefore, screening bioactive compounds for treatment of colon cancer is urgently needed. Sanguisorba officinalis L. (SO) has been demonstrated that the extractions or monomers possess potential anti-tumor effect. In this study, we firstly used cell membrane chromatography (CMC) and ultra-performance liquid chromatography coupled with (quadrupole) time-of-flight mass spectrometry (UHPLC-(Q) TOF-MS/MS) to identify a novel active ingredient, octyl gallate (OG), from SO methanol extract (SO-MtOH). HCT116 and SW620 cells lines were used for in vitro research, which showed OG presents great anti-colon cancer effect by inhibiting proliferation, inducing apoptosis, and repressing the migration and invasion. Furthermore, SW620 bearing athymic nude mice was used to investigate the potential antitumor activity in vivo, which exhibited OG treatment remarkably lessened the tumor volume. Mechanism studies showed that OG downregulated the PI3K/AKT/mTOR signaling axis and induced apoptosis by upregulating the Bax/Bcl-2 protein and the cleaved caspase-3, caspase-9. In conclusion, our research innovatively applied the method of CMC to intriguingly unearth the potential anti-colon cancer ingredient OG and demonstrated its the great antineoplastic activity, which provide a new insight for researchers efficiently developing the novel apoptosis-inducing compound for colon cancer therapy.
