Construction of a high-density SSR genetic linkage map and identification of QTL for storage-root yield and dry-matter content in sweetpotato
Chenxing Zheng,
Zhicheng Jiang,
Yusha Meng,
Jun Yu,
Xinsun Yang,
Huan Zhang,
Ning Zhao,
Shaozhen He,
Shaopei Gao,
Hong Zhai,
Qingchang Liu
Affiliations
Chenxing Zheng
Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China
Zhicheng Jiang
Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China
Yusha Meng
Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China; Institute of Rural Development, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China
Jun Yu
Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China
Xinsun Yang
Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei, China
Huan Zhang
Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China
Ning Zhao
Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China
Shaozhen He
Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China
Shaopei Gao
Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China
Hong Zhai
Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China; Corresponding authors.
Qingchang Liu
Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China; Corresponding authors.
Sweetpotato (Ipomoea batatas (L.) Lam.) is a widely grown food crop especially in developing countries. Increasing storage-root yield and dry-matter content has been the main breeding objective of the crop, and DNA marker-assisted breeding is needed for this purpose. In this study, using a mapping population of 500 F1 individuals from a cross between Xushu 18 (female) and Xu 781 (male), we constructed a high-density genetic linkage map of sweetpotato using 601 simple-sequence repeat (SSR) primer pairs. The Xushu 18 map contained 90 linkage groups with 5547 SSR markers and spanned 18,263.5 cM, and the Xu 781 map contained 90 linkage groups with 4599 SSR markers and spanned 18,043.7 cM, representing the highest genome coverage yet reported for sweetpotato. We identified 33 QTL for storage-root yield and 16 QTL for dry-matter content, explaining respectively 6.5%–47.5% and 3.2%–18.9% of variation. These results provide a foundation for fine-mapping and cloning of QTL and for marker-assisted breeding in sweetpotato.
