GAPDH controls extracellular vesicle biogenesis and enhances the therapeutic potential of EV mediated siRNA delivery to the brain

Ghulam Hassan Dar; Cláudia C. Mendes; Wei-Li Kuan; Alfina A. Speciale; Mariana Conceição; André Görgens; Inna Uliyakina; Miguel J. Lobo; Wooi F. Lim; Samir EL Andaloussi; Imre Mäger; Thomas C. Roberts; Roger A. Barker; Deborah C. I. Goberdhan; Clive Wilson; Matthew J. A. Wood

doi:10.1038/s41467-021-27056-3

Nature Communications (Nov 2021)

GAPDH controls extracellular vesicle biogenesis and enhances the therapeutic potential of EV mediated siRNA delivery to the brain

Ghulam Hassan Dar,
Cláudia C. Mendes,
Wei-Li Kuan,
Alfina A. Speciale,
Mariana Conceição,
André Görgens,
Inna Uliyakina,
Miguel J. Lobo,
Wooi F. Lim,
Samir EL Andaloussi,
Imre Mäger,
Thomas C. Roberts,
Roger A. Barker,
Deborah C. I. Goberdhan,
Clive Wilson,
Matthew J. A. Wood

Affiliations

Ghulam Hassan Dar: Department of Paediatrics, University of Oxford
Cláudia C. Mendes: Department of Physiology, Anatomy and Genetics, University of Oxford
Wei-Li Kuan: John van Geest Centre for Brain Repair, Department of Clinical Neurosciences, University of Cambridge
Alfina A. Speciale: Department of Paediatrics, University of Oxford
Mariana Conceição: Department of Paediatrics, University of Oxford
André Görgens: Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet
Inna Uliyakina: Department of Physiology, Anatomy and Genetics, University of Oxford
Miguel J. Lobo: Department of Physiology, Anatomy and Genetics, University of Oxford
Wooi F. Lim: Department of Paediatrics, University of Oxford
Samir EL Andaloussi: Department of Laboratory Medicine, Clinical Research Center, Karolinska Institutet
Imre Mäger: Department of Paediatrics, University of Oxford
Thomas C. Roberts: Department of Paediatrics, University of Oxford
Roger A. Barker: John van Geest Centre for Brain Repair, Department of Clinical Neurosciences, University of Cambridge
Deborah C. I. Goberdhan: Department of Physiology, Anatomy and Genetics, University of Oxford
Clive Wilson: Department of Physiology, Anatomy and Genetics, University of Oxford
Matthew J. A. Wood: Department of Paediatrics, University of Oxford

DOI: https://doi.org/10.1038/s41467-021-27056-3
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 15

Abstract

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GAPDH is generally considered a housekeeping gene and functions in glycolysis. Here, the authors show that GAPDH has a role in promoting vesicle clustering in endosomes and can load siRNA onto the surface of extracellular vesicles, which can be exploited therapeutically.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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