Mixed Pluronic—Cremophor Polymeric Micelles as Nanocarriers for Poorly Soluble Antibiotics—The Influence on the Antibacterial Activity
Maria Antonia Tănase,
Adina Raducan,
Petruţa Oancea,
Lia Mara Diţu,
Miruna Stan,
Cristian Petcu,
Cristina Scomoroşcenco,
Claudia Mihaela Ninciuleanu,
Cristina Lavinia Nistor,
Ludmila Otilia Cinteza
Affiliations
Maria Antonia Tănase
Physical Chemistry Department, University of Bucharest, 030018 Bucharest, Romania
Adina Raducan
Physical Chemistry Department, University of Bucharest, 030018 Bucharest, Romania
Petruţa Oancea
Physical Chemistry Department, University of Bucharest, 030018 Bucharest, Romania
Lia Mara Diţu
Microbiology Department, Faculty of Biology, University of Bucharest, 60101 Bucharest, Romania
Miruna Stan
Department of Biochemistry and Molecular Biology, Faculty of Biology, ICUB-Research Institute of the University of Bucharest, University of Bucharest, 050095 Bucharest, Romania
Cristian Petcu
National Institute for Research and Development in Chemistry and Petrochemistry-ICECHIM, Polymer Department, 202 Spl. Independentei, 060021 Bucharest, Romania
Cristina Scomoroşcenco
National Institute for Research and Development in Chemistry and Petrochemistry-ICECHIM, Polymer Department, 202 Spl. Independentei, 060021 Bucharest, Romania
Claudia Mihaela Ninciuleanu
National Institute for Research and Development in Chemistry and Petrochemistry-ICECHIM, Polymer Department, 202 Spl. Independentei, 060021 Bucharest, Romania
Cristina Lavinia Nistor
National Institute for Research and Development in Chemistry and Petrochemistry-ICECHIM, Polymer Department, 202 Spl. Independentei, 060021 Bucharest, Romania
Ludmila Otilia Cinteza
Physical Chemistry Department, University of Bucharest, 030018 Bucharest, Romania
In this work, novel polymeric mixed micelles from Pluronic F127 and Cremophor EL were investigated as drug delivery systems for Norfloxacin as model antibiotic drug. The optimal molar ratio of surfactants was determined, in order to decrease critical micellar concentration (CMC) and prepare carriers with minimal surfactant concentrations. The particle size, zeta potential, and encapsulation efficiency were determined for both pure and mixed micelles with selected composition. In vitro release kinetics of Norfloxacin from micelles show that the composition of surfactant mixture generates tunable extended release. The mixed micelles exhibit good biocompatibility against normal fibroblasts MRC-5 cells, while some cytotoxicity was found in all micellar systems at high concentrations. The influence of the surfactant components in the carrier on the antibacterial properties of Norfloxacin was investigated. The drug loaded mixed micellar formulation exhibit good activity against clinical isolated strains, compared with the CLSI recommended standard strains (Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922). P. aeruginosa 5399 clinical strain shows low sensitivity to Norfloxacin in all tested micelle systems. The results suggest that Cremophor EL-Pluronic F127 mixed micelles can be considered as novel controlled release delivery systems for hydrophobic antimicrobial drugs.
