(INVITED)Nanocoated fiber label-free biosensing for perfluorooctanoic acid detection by lossy mode resonance

Giulia Moro; Francesco Chiavaioli; Stefano Liberi; Pablo Zubiate; Ignacio Del Villar; Alessandro Angelini; Karolien De Wael; Francesco Baldini; Ligia Maria Moretto; Ambra Giannetti

Results in Optics (Dec 2021)

(INVITED)Nanocoated fiber label-free biosensing for perfluorooctanoic acid detection by lossy mode resonance

Giulia Moro,
Francesco Chiavaioli,
Stefano Liberi,
Pablo Zubiate,
Ignacio Del Villar,
Alessandro Angelini,
Karolien De Wael,
Francesco Baldini,
Ligia Maria Moretto,
Ambra Giannetti

Affiliations

Giulia Moro: Department of Molecular Sciences and Nanosystems, Ca’ Foscari University of Venice, Via Torino 155, 30172 Mestre, Italy; AXES Research Group, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium; NanoLab Center of Excellence, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium
Francesco Chiavaioli: Institute of Applied Physics “Nello Carrara”, National Research Council of Italy (CNR), 50019 Sesto Fiorentino, Firenze, Italy; Corresponding author.
Stefano Liberi: Department of Biology, University of Padua, Via U. Bassi 58, 35131 Padova, Italy
Pablo Zubiate: Electrical and Electronic Engineering Department, Public University of Navarra, 31006 Pamplona, Spain
Ignacio Del Villar: Electrical and Electronic Engineering Department, Public University of Navarra, 31006 Pamplona, Spain; Institute of Smart Cities (ISC), Public University of Navarra, 31006 Pamplona, Spain
Alessandro Angelini: Department of Molecular Sciences and Nanosystems, Ca’ Foscari University of Venice, Via Torino 155, 30172 Mestre, Italy; European Centre for Living Technology (ECLT), Ca’ Bottacin, Dorsoduro 3911, Calle Crosera, 30123 Venice, Italy
Karolien De Wael: AXES Research Group, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium; NanoLab Center of Excellence, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium
Francesco Baldini: Institute of Applied Physics “Nello Carrara”, National Research Council of Italy (CNR), 50019 Sesto Fiorentino, Firenze, Italy
Ligia Maria Moretto: Department of Molecular Sciences and Nanosystems, Ca’ Foscari University of Venice, Via Torino 155, 30172 Mestre, Italy
Ambra Giannetti: Institute of Applied Physics “Nello Carrara”, National Research Council of Italy (CNR), 50019 Sesto Fiorentino, Firenze, Italy

Journal volume & issue: Vol. 5
p. 100123

Abstract

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The determination of per- and polyfluoroalkyl substances (PFAS) in environmental samples, such as drinking waters, requires the design of high performing and versatile sensing strategies. Label-free biosensing platforms based on specialty fiber optics are a valid option to face this challenge. Among them, lossy mode resonance (LMR) fiber optic biosensors are showing remarkable performance in terms of detection limit, selectivity, and reproducibility. The detection of small molecules, such as perfluorooctanoic acid (PFOA), can be achieved with the help of well-designed biological recognition layers. In this study, the biosensing potentialities of a label-free LMR-assisted optical platform based on nanocoated fibers are investigated. Delipidated human serum albumin (hSA) was used as biological recognition layer for PFOA in aqueous solution. Different fiber functionalization protocols based on the covalent immobilization of hSA were tested. The conformational changes related to the formation of hSA/PFOA complex were followed via optical monitoring of LMR spectral shift, showing a trend that can be modeled with Langmuir adsorption isotherm. These results confirmed the potentiality of LMR-based fiber biosensors for the detection of small molecules, such as PFOA, in synthetic samples.

Published in Results in Optics

ISSN: 2666-9501 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Physics: Optics. Light
Website: https://www.journals.elsevier.com/results-in-optics

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