Nature Communications (Apr 2020)

Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites

  • Nikolaus A. Watson,
  • Tyrell N. Cartwright,
  • Conor Lawless,
  • Marcos Cámara-Donoso,
  • Onur Sen,
  • Kosuke Sako,
  • Toru Hirota,
  • Hiroshi Kimura,
  • Jonathan M. G. Higgins

DOI
https://doi.org/10.1038/s41467-020-15428-0
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 16

Abstract

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Identifying kinases responsible for specific phosphorylation events remains challenging. Here, the authors leverage kinase inhibitor profiles for the identification of kinase-substrate site pairs in cell extracts, developing a method that can identify the enzymes responsible for unassigned phosphorylation events.