Simulated vestibular spatial disorientation mouse model under coupled rotation revealing potential involvement of Slc17a6
Tong Chang,
Min Zhang,
Jing Zhu,
Han Wang,
Cong-cong Li,
Kan Wu,
Zhuo-ru Zhang,
Yi-hong Jiang,
Fei Wang,
Hao-tian Wang,
Xiao-Cheng Wang,
Yong Liu
Affiliations
Tong Chang
Center of Clinical Aerospace Medicine, School of Aerospace Medicine, Key Laboratory of Aerospace Medicine of Ministry of Education, Air Force Medical University, Xi’an 710032, China; Department of Aviation Medicine, The First Affiliated Hospital, Air Force Medical University, Xi’an 710032, China
Min Zhang
Center of Clinical Aerospace Medicine, School of Aerospace Medicine, Key Laboratory of Aerospace Medicine of Ministry of Education, Air Force Medical University, Xi’an 710032, China; Department of Aviation Medicine, The First Affiliated Hospital, Air Force Medical University, Xi’an 710032, China
Jing Zhu
Center of Clinical Aerospace Medicine, School of Aerospace Medicine, Key Laboratory of Aerospace Medicine of Ministry of Education, Air Force Medical University, Xi’an 710032, China; Department of Aviation Medicine, The First Affiliated Hospital, Air Force Medical University, Xi’an 710032, China
Han Wang
Center of Clinical Aerospace Medicine, School of Aerospace Medicine, Key Laboratory of Aerospace Medicine of Ministry of Education, Air Force Medical University, Xi’an 710032, China; Department of Aviation Medicine, The First Affiliated Hospital, Air Force Medical University, Xi’an 710032, China
Cong-cong Li
Center of Clinical Aerospace Medicine, School of Aerospace Medicine, Key Laboratory of Aerospace Medicine of Ministry of Education, Air Force Medical University, Xi’an 710032, China; Department of Aviation Medicine, The First Affiliated Hospital, Air Force Medical University, Xi’an 710032, China
Kan Wu
Center of Clinical Aerospace Medicine, School of Aerospace Medicine, Key Laboratory of Aerospace Medicine of Ministry of Education, Air Force Medical University, Xi’an 710032, China; Department of Aviation Medicine, The First Affiliated Hospital, Air Force Medical University, Xi’an 710032, China
Zhuo-ru Zhang
Center of Clinical Aerospace Medicine, School of Aerospace Medicine, Key Laboratory of Aerospace Medicine of Ministry of Education, Air Force Medical University, Xi’an 710032, China; Department of Aviation Medicine, The First Affiliated Hospital, Air Force Medical University, Xi’an 710032, China
Yi-hong Jiang
Center of Clinical Aerospace Medicine, School of Aerospace Medicine, Key Laboratory of Aerospace Medicine of Ministry of Education, Air Force Medical University, Xi’an 710032, China; Department of Aviation Medicine, The First Affiliated Hospital, Air Force Medical University, Xi’an 710032, China
Fei Wang
School of Basic Medicine, Air Force Medical University, Xi’an 710032, China
Hao-tian Wang
School of Basic Medicine, Air Force Medical University, Xi’an 710032, China
Xiao-Cheng Wang
Center of Clinical Aerospace Medicine, School of Aerospace Medicine, Key Laboratory of Aerospace Medicine of Ministry of Education, Air Force Medical University, Xi’an 710032, China; Department of Aviation Medicine, The First Affiliated Hospital, Air Force Medical University, Xi’an 710032, China; Corresponding author
Yong Liu
School of Aerospace Medicine, Key Laboratory of Aerospace Medicine of Ministry of Education, Air Force Medical University, Xi’an 710032, China; Corresponding author
Summary: Spatial disorientation (SD) is the main contributor to flight safety risks, but research progress in animals has been limited, impeding a deeper understanding of the underlying mechanisms of SD. This study proposed a method for constructing and evaluating a vestibular SD mouse model, which adopted coupled rotational stimulation with visual occlusion. Physiological parameters were measured alongside behavioral indices to assess the model, and neuronal changes were observed through immunofluorescent staining. The evaluation of the model involved observing decreased colonic temperature and increased arterial blood pressure in mice exposed to SD, along with notable impairments in motor and cognitive function. Our investigation unveiled that vestibular SD stimulation elicited neuronal activation in spatially associated cerebral areas, such as the hippocampus. Furthermore, transcriptomic sequencing and bioinformatics analysis revealed the potential involvement of Slc17a6 in the mechanism of SD. These findings lay a foundation for further investigation into the molecular mechanisms underlying SD.
