The comparative analysis of the gene expression profiles of IL-1β, COX-2 and Collagenase type II in inflamed dental pulps

Abstract

The aim of the present study was to detect the gene expression profiles of IL-1β, COX-2 and Collagenase Type II in dental pulps. Further, the study aimed at comparison of these profiles in healthy and inflamed dental pulps. Gene expression was assessed using Reverse Transcriptase Reaction and Real-Time-PCR. The results of the present study showed significant increase of the gene expression of IL-1β (p = 0,001), COX-2 (p = 0,002) in the inflamed samples, if compared with healthy pulps. Any statistical differences between the gene expression of Collagenase Type II (p = 0,757) in inflamed and healthy dental pulps could not be detected.

Keywords

• медиаторы воспаления
• количественная полимеразная цепная реакция
• экспресс-тест
• необратимый пульпит
• диагностика
• cytokines
• real-time pcr
• chairside test
• irreversible pulpitis
• diagnostics