Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber

Amy G. Harris; Vico Schot; Michele Carrabba; Dominga Iacobazzi; Mohamed T. Ghorbel; James P.K. Armstrong; Adam W. Perriman; Massimo Caputo; Giovanni Biglino; Francesca Bartoli-Leonard

STAR Protocols (Mar 2024)

Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber

Amy G. Harris,
Vico Schot,
Michele Carrabba,
Dominga Iacobazzi,
Mohamed T. Ghorbel,
James P.K. Armstrong,
Adam W. Perriman,
Massimo Caputo,
Giovanni Biglino,
Francesca Bartoli-Leonard

Affiliations

Amy G. Harris: Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK; Corresponding author
Vico Schot: Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK
Michele Carrabba: Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK
Dominga Iacobazzi: Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK
Mohamed T. Ghorbel: Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK
James P.K. Armstrong: Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK
Adam W. Perriman: School of Cellular and Molecular Medicine, Biomedical Sciences Building, University Walk, BS8 1TD Bristol, UK
Massimo Caputo: Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK; Cardiac Surgery, University Hospitals Bristol, NHS Foundation Trust, BS2 8HW Bristol, UK
Giovanni Biglino: Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK; Cardiorespiratory Unit, Great Ormond Street Hospital for Children, NHS Foundation Trust, WC1N 3JH London, UK
Francesca Bartoli-Leonard: Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK; Corresponding author

Journal volume & issue: Vol. 5, no. 1
p. 102899

Abstract

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Summary: Surgical treatment of pediatric congenital heart disease with tissue grafts is a lifesaving intervention. Decellularization to reduce immunogenicity of tissue grafts is an increasingly popular alternative to glutaraldehyde fixation. Here, we present a protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber. We describe steps for 3D printing the flow rig, preparing porcine tissue, and using the flow rig to utilize shear forces for decellularization. We then detail procedures for characterizing the acellular scaffold.For complete details on the use and execution of this protocol, please refer to Vafaee et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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