One Health (Dec 2024)
Broad range molecular detection methods identify only Borrelia spp. in erythema migrans biopsies and blood of tick-bitten patients
- Philippe Pérot,
- Laura Tondeur,
- Sara Moutailler,
- Delphine Chrétien,
- Nicole Corre-Catelin,
- Muriel Vayssier-Taussat,
- Marc Eloit,
- Catherine Chirouze,
- Céline Cazorla,
- Laurence Arowas,
- Elisabeth Botelho-Nevers,
- Céline Cazorla,
- Catherine Chirouze,
- Delphine Chrétien,
- Nicole Corre-Catelin,
- Marc Eloit,
- Pascale Frey-Klett,
- Arnaud Fontanet,
- Clémence Galon,
- Karine Lacombe,
- Sara Moutailler,
- Philippe Pérot,
- Véronique Perronne,
- Valentine Piquard,
- Marie Préau,
- Costanza Puppo,
- Laura Tondeur,
- Marie-Noelle Ungeheuer,
- Muriel Vayssier-Taussat,
- Ayla Zayoud
Affiliations
- Philippe Pérot
- Pathogen Discovery Laboratory, Institut Pasteur, Université Paris Cité, F-75015 Paris, France; Institut Pasteur, Université Paris Cité, The WOAH (OIE) Collaborating Center for the Detection and Identification in Humans of Emerging Animal Pathogens, F-75015 Paris, France; Corresponding author at: Institut Pasteur, Pathogen Discovery Laboratory, 25-28 rue du Dr. Roux, 75015 Paris, France.
- Laura Tondeur
- Emerging Infectious Diseases Unit, Institut Pasteur, Paris, France
- Sara Moutailler
- ANSES, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, F-94700, France
- Delphine Chrétien
- Pathogen Discovery Laboratory, Institut Pasteur, Université Paris Cité, F-75015 Paris, France; Institut Pasteur, Université Paris Cité, The WOAH (OIE) Collaborating Center for the Detection and Identification in Humans of Emerging Animal Pathogens, F-75015 Paris, France
- Nicole Corre-Catelin
- Institut Pasteur, Université Paris Cité, Clinical Investigation and Access to Research Bioresources (ICAReB) Platform, Paris, France
- Muriel Vayssier-Taussat
- Animal Health Division, INRAE, 37380 Nouzilly, France
- Marc Eloit
- Pathogen Discovery Laboratory, Institut Pasteur, Université Paris Cité, F-75015 Paris, France; Institut Pasteur, Université Paris Cité, The WOAH (OIE) Collaborating Center for the Detection and Identification in Humans of Emerging Animal Pathogens, F-75015 Paris, France; Ecole Nationale Vétérinaire d'Alfort, F-94700 Maisons-Alfort, France
- Catherine Chirouze
- Chrono-Environnement UMR6249, CNRS, Université Bourgogne Franche-Comté, F-25000 Besançon, France; Infectious Disease Department, University Hospital of Besançon, F-25000, France
- Céline Cazorla
- Infectious Disease department, University Hospital of Saint Etienne, Avenue Albert-Raimond, 42055, Saint Etienne Cedex 02, France
- Laurence Arowas
- Elisabeth Botelho-Nevers
- Céline Cazorla
- Catherine Chirouze
- Delphine Chrétien
- Nicole Corre-Catelin
- Marc Eloit
- Pascale Frey-Klett
- Arnaud Fontanet
- Clémence Galon
- Karine Lacombe
- Sara Moutailler
- Philippe Pérot
- Véronique Perronne
- Valentine Piquard
- Marie Préau
- Costanza Puppo
- Laura Tondeur
- Marie-Noelle Ungeheuer
- Muriel Vayssier-Taussat
- Ayla Zayoud
- Journal volume & issue
-
Vol. 19
p. 100886
Abstract
In this multicenter study conducted in France, we challenged the hypothesis of the transmission of pathogens other than Borrelia spp. in 22 patients developing erythema migrans following a tick bite. Using a combination of high-throughput microfluidic PCRs and agnostic metagenomics on skin biopsies and blood samples, no microorganisms other than Borrelia spp. was found.
