Experimental Justification of Using Aseptisorb-A and Platelet-Rich Plasma in Endoscopic Treatment of Mold Bleeding Stomach Defects

Abstract

The aim of this study was to investigate the possibility of applying the biologically active draining sorbent Aseptisorb-A in combination with platelet-rich plasma (PRP) to arrest bleeding of mold stomach defects in dogs. Methods and Results: The experimental study was done on 12 outbred dogs (both sexes). Fibrogastroduodenoscopy (FGDS) was performed on all animals under intravenous anesthesia. During FGDS, two ulcerative defects (pilot and control) were made in the antrum of the stomach at 4-5cm distant from each other. Endoscopic hemostasis in pilot ulcers was achieved with the help of pneumatic insufflation of powder-like Aseptisorb-A (0.3mg) on the bleeding defect with further application of platelet-rich autologous plasma from the animal. Endoscopic treatment of control ulcers was not done; such ulcers were used to estimate the time of spontaneous hemostasis. It was determined that in pilot ulcers after described interventions, bleeding arrest occurred in 3.0|2.5|4.0 sec (Ме | upper quartile | lower quartile) (p<0.01). It was noted that in all pilot ulcers, hemostasis was definitive and there was no recurrence of bleeding. In the control ulcers, bleeding arrest occurred in 29.0|27.5|30.5 sec (p≤0.01). In endoscopic gastroscopy, two cases of the reinitiation of haemorrhages in the form of hematin on ulcers were fixed. The reparative process in pilot ulcers treated with Aseptisorb-A and PRP occurred quicker and more efficiently. Complete healing of pilot ulcers occurred in 8.0|8.0|8.5 days (p≤0.01) with formation of a slight sword-cut, which did not destroy the wall of the organ. Complete healing of control ulcers was identified in 15.0|15.0|16.0 days (P<0.01) with formation of a rough scar, which deformed the organ’s wall. Conclusion: Using the biologically active draining sorbent Aseptisorb-A in combination with PRP in endoscopic treatment of mold bleeding in the defects of stomachs accelerates the reparative process, reduces the time of healing in experimental ulcers, improves the quality of healing and does not damage stomach tissue.

Keywords

• mold bleeding
• endoscopic hemostasis
• Aseptisorb-A
• platelet-rich plasma
• reparative process