A growth-regulating factor 7 (GRF7)-mediated gene regulatory network promotes leaf growth and expansion in sugarcane
Qiaoyu Wang,
Yihan Li,
Dadong Lin,
Xiaoxi Feng,
Yongjun Wang,
Tianyou Wang,
Hongyan Ding,
Jisen Zhang
Affiliations
Qiaoyu Wang
State Key Lab for Conservation and Utilization of Subtropical AgroBiological Resources and Guangxi Key Lab for Saccharum Biology, Guangxi University, Nanning 530005, Guangxi, China; Center for Genomics and Biotechnology/Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology/Key Laboratory of Saccharum Biology and Genetic Breeding/National Engineering Research Center for Saccharum, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
Yihan Li
State Key Lab for Conservation and Utilization of Subtropical AgroBiological Resources and Guangxi Key Lab for Saccharum Biology, Guangxi University, Nanning 530005, Guangxi, China
Dadong Lin
Center for Genomics and Biotechnology/Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology/Key Laboratory of Saccharum Biology and Genetic Breeding/National Engineering Research Center for Saccharum, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
Xiaoxi Feng
Center for Genomics and Biotechnology/Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology/Key Laboratory of Saccharum Biology and Genetic Breeding/National Engineering Research Center for Saccharum, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
Yongjun Wang
Center for Genomics and Biotechnology/Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology/Key Laboratory of Saccharum Biology and Genetic Breeding/National Engineering Research Center for Saccharum, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
Tianyou Wang
State Key Lab for Conservation and Utilization of Subtropical AgroBiological Resources and Guangxi Key Lab for Saccharum Biology, Guangxi University, Nanning 530005, Guangxi, China
Hongyan Ding
State Key Lab for Conservation and Utilization of Subtropical AgroBiological Resources and Guangxi Key Lab for Saccharum Biology, Guangxi University, Nanning 530005, Guangxi, China
Jisen Zhang
State Key Lab for Conservation and Utilization of Subtropical AgroBiological Resources and Guangxi Key Lab for Saccharum Biology, Guangxi University, Nanning 530005, Guangxi, China; Center for Genomics and Biotechnology/Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology/Key Laboratory of Saccharum Biology and Genetic Breeding/National Engineering Research Center for Saccharum, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China; Corresponding author.
Knowledge of the function of growth-regulating factors (GRFs) in sugarcane (Saccharum officinarum and S. spontaneum) growth and development could assist breeders in selecting desirable plant architectures. However, limited information about GRFs is available in Saccharum due to their polyploidy. In this study, 22 GRFs were identified in the two species and their conserved domains, gene structures, chromosome location, and synteny were characterized. GRF7 expression varied among tissues and responded to diurnal rhythm. SsGRF7-YFP was localized preferentially in the nucleus and appears to act as a transcriptional cofactor. SsGRF7 positively regulated the size and length of rice leaves, possibly by regulating cell size and plant hormones. Of seven potential transcription factors binding to the SsGRF7 promoter in S. spontaneum, four showed positive expression patterns, and two showed negative expression patterns relative to SsGRF7.
