Journal of Extracellular Vesicles (Dec 2021)
Updating MISEV: Evolving the minimal requirements for studies of extracellular vesicles
- Kenneth W Witwer,
- Deborah CI Goberdhan,
- Lorraine O'Driscoll,
- Clotilde Théry,
- Joshua A Welsh,
- Cherie Blenkiron,
- Edit I Buzás,
- Dolores Di Vizio,
- Uta Erdbrügger,
- Juan M Falcón‐Pérez,
- Qing‐Ling Fu,
- Andrew F Hill,
- Metka Lenassi,
- Jan Lötvall,
- Rienk Nieuwland,
- Takahiro Ochiya,
- Sophie Rome,
- Susmita Sahoo,
- Lei Zheng
Affiliations
- Kenneth W Witwer
- Departments of Molecular and Comparative Pathobiology and Neurology and The Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease Johns Hopkins University School of Medicine Baltimore Maryland USA
- Deborah CI Goberdhan
- Department of Physiology Anatomy and Genetics University of Oxford Oxford UK
- Lorraine O'Driscoll
- School of Pharmacy and Pharmaceutical Sciences Panoz Institute and Trinity Biomedical Sciences Institute (TBSI) & Trinity St. James's Cancer Institute (TSJCI) Trinity College Dublin Dublin Ireland
- Clotilde Théry
- INSERM U932, Institut Curie PSL Research University Paris France
- Joshua A Welsh
- Translational Nanobiology Section Laboratory of Pathology Center for Cancer Research National Cancer Institute National Institutes of Health Bethesda Maryland USA
- Cherie Blenkiron
- Department of Molecular Medicine and Pathology The University of Auckland Auckland New Zealand
- Edit I Buzás
- Department of Genetics Cell‐ and Immunobiology HCEMM‐SU Extracellular Vesicles Research Group, and ELKH‐SE Immune‐Proteogenomics Extracellular Vesicles Research Group Semmelweis University Budapest Hungary
- Dolores Di Vizio
- Department of Surgery Department of Pathology & Laboratory Medicine Samuel Oschin Comprehensive Cancer Institute Cedars‐Sinai Medical Center Division of Cancer Biology and Therapeutics Los Angeles California USA
- Uta Erdbrügger
- Department of Medicine Division of Nephrology University of Virginia Charlottesville Virginia USA
- Juan M Falcón‐Pérez
- Exosomes Laboratory & Metabolomics Platf CIC bioGUNE‐BRTA IKERBASQUE, CIBERehd Bilbao Spain
- Qing‐Ling Fu
- Exosome Research and Translational Center The First Affiliated Hospital Otorhinolaryngology Hospital Sun Yat‐sen University Guangzhou China
- Andrew F Hill
- Department of Biochemistry and Genetics La Trobe Institute for Molecular Science La Trobe University Bundoora VIC Australia
- Metka Lenassi
- Institute of Biochemistry and Molecular Genetics Faculty of Medicine University of Ljubljana Ljubljana Slovenia
- Jan Lötvall
- Krefting Research Centre University of Gothenburg Göteborg Sweden
- Rienk Nieuwland
- Laboratory of Experimental Clinical Chemistry and Vesicle Observation Center Amsterdam University Medical Center University of Amsterdam Amsterdam The Netherlands
- Takahiro Ochiya
- Department of Molecular and Cellular Medicine Tokyo Medical University Tokyo Japan
- Sophie Rome
- CarMeN Laboratory (INSERM 1060, INRAE 1397) University of Lyon & Faculty of Medicine Lyon‐Sud Pierre‐Bénite France
- Susmita Sahoo
- Cardiovascular Research Institute Icahn School of Medicine at Mount Sinai New York New York USA
- Lei Zheng
- Department of Laboratory Medicine Nanfang Hospital Southern Medical University Guangzhou China
- DOI
- https://doi.org/10.1002/jev2.12182
- Journal volume & issue
-
Vol. 10,
no. 14
pp. n/a – n/a
Abstract
Abstract The minimal information for studies of extracellular vesicles (EVs, MISEV) is a field‐consensus rigour initiative of the International Society for Extracellular Vesicles (ISEV). The last update to MISEV, MISEV2018, was informed by input from more than 400 scientists and made recommendations in the six broad topics of EV nomenclature, sample collection and pre‐processing, EV separation and concentration, characterization, functional studies, and reporting requirements/exceptions. To gather opinions on MISEV and ideas for new updates, the ISEV Board of Directors canvassed previous MISEV authors and society members. Here, we share conclusions that are relevant to the ongoing evolution of the MISEV initiative and other ISEV rigour and standardization efforts.
Keywords
