Biallelic and gene-wide genomic substitution for endogenous intron and retroelement mutagenesis in human cells

Abstract

Functional annotation of the vast noncoding landscape of the diploid human genome still remains a major challenge of genomic research. Here the authors present a scarless, biallelic, and 100 kb-scale mutagenesis in human cells that uncovers functional significances of endogenous introns and retrotransposons in the chromatin context.