Journal of Animal Reproduction and Biotechnology (Dec 2015)

Motility, Fertilizability and Subsequent Embryonic Development of Frozen-thawed Spermatozoa derived from Epididymis in Hanwoo

  • Byoung-Chul Yang,
  • Sung-Sik Kang,
  • Chang-Seok Park,
  • Ui-Hyung Kim,
  • Hyeong-Cheol Kim,
  • Gi-Jun Jeon,
  • Sidong Kim,
  • Seok-Dong Lee,
  • Hyun-Jae Lee,
  • Sang-Rae Cho

DOI
https://doi.org/10.12750/JET.2015.30.4.271
Journal volume & issue
Vol. 30, no. 4
pp. 271 – 276

Abstract

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The aim of the study was to investigate the ability of sperm derived from the epididymis in regard to sperm motility, sperm penetration to oocyte and subsequent development of the embryo. Frozen-thawed sperm from epididymis showed similar percentage of motile sperm (VSL ≥ 25 μm/sec) as compared to that of commercial sperm (control). Sperm penetration of frozen-thawed epididymal and commercial sperm was not significantly different. Moreover, cleavage and blastocyst rates were similar in both epididymal and control. Sperm derived from the epididymis also showed fertilizability and subsequent embryonic development

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