Ho Chi Minh City Open University Journal of Science - Engineering and Technology (Sep 2018)

Construction of a dataset for the gene nrLSU to support the identification of cantharellus at Langbian Mountain, Lam Dong

  • Trinh Van Hanh,
  • Vu Tien Luyen,
  • Pham Huu Hung,
  • Truong Binh Nguyen,
  • Tran Duc Vinh,
  • Lao Duc Thuan,
  • Le Huyen Ai Thuy

DOI
https://doi.org/10.46223/HCMCOUJS.tech.en.8.2.342.2018
Journal volume & issue
Vol. 8, no. 2
pp. 14 – 21

Abstract

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Fungal species of Cantharellales order are found worldwide including Europe, Africa, Asia, and North America. This clade includes two smaller, separate, but closely related genera Cantharellus and Craterellus. Cantharellus was firstly identified in the middle of the 17th century. Besides traditional classification based on morphology, molecular phylogeny of the nuclear ribosomal large subunit gene (nrLSU) can be an effective tool to provide more information during species identification for this group. In this current report, a dataset of the nrLSU gene including 60 sequences of Cantharellus species and 1 sequence belonging to Craterellus (outgroup), was obtained from Genbank. Phylogenetic analyses by MEGA 6.0 showed a monophyletic relationship between the six well-established clades, namely Cantharellus (Clade 1), Rubrinus (Clade 2), Cinnabarinus (Clade 3), Parvocantharellus (Clade 4), Pseudocantharellus (Clade 5) and subgenus Afrocantharellus (Clade 6) similar to that of morphological classification. Therefore, this dataset is efficient to support the identification of the fungal samples, collected at the Langbian Mountain, Lam Dong.

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